Anti-POLR2B Antibody Picoband® Fluoro647 Conjugated
Product Specifications
Background
DNA-ed RNA polymerase II subunit RPB2 is an enzyme that in humans is encoded by the POLR2B gene. This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants.
Synonyms
RecQ-mediated genome instability protein 2; hRMI2; BLM-associated protein of 18 kDa; BLAP18; RMI2; C16orf75
Gene Name
POLR2B
Gene ID
5431
UniProt
P30876
Host
Rabbit
Reactivity
Human, Mouse, Rat
Cross Reactivity
No cross-reactivity with other proteins.
Immunogen
E.coli-derived human POLR2B recombinant protein (Position: E49-Q1145) .
Clonality
Polyclonal
Applications
Flow Cytometry
Field of Research
Chromatin Binding Proteins, DNA/RNA, DNA/RNA Binding, DNA Damage & Repair, Epigenetics and Nuclear Signaling
Purification
Immunogen affinity purified.
Form
Liquid
Function
Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates. It is required to regulate sister chromatid segregation and to limit DNA crossover. Essential for the stability, localization, and function of BLM, TOP3A, and complexes containing BLM. In the RMI complex, it is required to target BLM to chromatin and stress-induced nuclear foci and mitotic phosphorylation of BLM.
References & Citations
1. Acker, J., Mattei, M.-G., Wintzerith, M., Roeckel, N., Depetris, D., Vigneron, M., Kedinger, C. Chromosomal localization of human RNA polymerase II subunit genes. Genomics 20: 496-499, 1994. 2. Acker, J., Wintzerith, M., Vigneron, M., Kedinger, C. Primary structure of the second largest subunit of human RNA polymerase II (or B) . J. Molec. Biol. 226: 1295-1299, 1992. 3. Hsin, J.-P., Sheth, A., Manley, J. L. RNAP II CTD phosphorylated on threonine-4 is required for histone mRNA 3-prime end processing. Science 334: 683-686, 2011.
Storage Conditions
At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Applications Notes
6
Gene Name Synonym
RecQ mediated genome instability 2
Subcellular Location
Nucleus.
Isotype
Rabbit IgG
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
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