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Anti-MARCHF2 Antibody Picoband® Fluoro488 Conjugated

Product Specifications

Background

MARCH2 is an E3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC and CD86, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then ly transfer the ubiquitin to targeted substrates. MARCH2 may be involved in endosomal trafficking through interaction with STX6.

Synonyms

RNA-binding protein 47; RNA-binding motif protein 47; RBM47

Gene Name

MARCHF2

Gene ID

51257

UniProt

Q9P0N8

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human MARCHF2 recombinant protein (Position: M1-V246) .

Clonality

Polyclonal

Tissue Specificity

Abundantly expressed in tonsil, lymph node, and trachea; strong expression in prostate; lower expression in thyroid, stomach, and colon. .

Applications

Flow Cytometry

Field of Research

Chromatin Binding Proteins, DNA/RNA Binding, Epigenetics and Nuclear Signaling

Purification

Immunogen affinity purified.

Form

Liquid

Function

Can bind to the surface of B-lymphoma cells, but not T- lymphoma cells, consistent with a function as a secreted mediator acting upon B-cells.

References & Citations

1. Bartee, E., Mansouri, M., Nerenberg, B. T. H., Gouveia, K., Fruh, K. Downregulation of major histocompatibility complex class I by human ubiquitin ligases related to viral immune evasion proteins. J. Virology 78: 1109-1120, 2004. 2. De Gassart, A., Camosseto, V., Thibodeau, J., Ceppi, M., Catalan, N., Pierre, P., Gatti, E. MHC class II stabilization at the surface of human dendritic cells is the result of maturation-dependent MARCH I down-regulation. Proc. Nat. Acad. Sci. 105: 3491-3496, 2008. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 3/29/2010.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Calculated Molecular Weight

64099 MW

Applications Notes

6

Gene Name Synonym

RNA-binding protein 47

Subcellular Location

Nucleus .

Protein Name

Follicular dendritic cell secreted peptide

Isotype

Rabbit IgG

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

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