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Anti-NCOA5 Antibody Picoband®

Boster Bio Anti-NCOA5 Antibody Picoband® catalog # A07810-2. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Nuclear receptor coactivator 5 (NCOA5), also known as coactivator independent of AF-2 function (CIA), is a protein that in humans is encoded by the NCOA5 gene. This gene encodes a coregulator for the alpha and beta estrogen receptors and the orphan nuclear receptor NR1D2. The protein localizes to the nucleus, and is thought to have both coactivator and corepressor functions. Its interaction with nuclear receptors is independent of the AF2 domain on the receptors, which is known to regulate interaction with other coreceptors. Several alternatively spliced transcript variants have been found for this gene.

Synonyms

Protein SOX-15; Protein SOX-12; Protein SOX-20; SOX15; SOX12; SOX20; SOX26; SOX27

Gene Name

NCOA5

Gene ID

57727

UniProt

Q9HCD5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human NCOA5 recombinant protein (Position: R191-Q560) .

Clonality

Polyclonal

Tissue Specificity

Widely expressed in fetal and adult tissues examined, highest level found in fetal spinal cord and adult brain and testis.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Golgi Proteins, Protein Trafficking, Signal Transduction, Vesicle Transport

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Binds to the 5'-AACAAT-3' sequence.

References & Citations

1. Gao, S., Li, A., Liu, F., Chen, F., Williams, M., zhang, C., Kelley, Z., Wu, C.-L., Luo, R., Xiao, H. NCOA5 haploinsufficiency results in glucose intolerance and subsequent hepatocellular carcinoma. Cancer Cell 24: 725-737, 2013. 2. Sauve, F., McBroom, L. D. B., Gallant, J., Moraitis, A. N., Labrie, F., Giguere, V. CIA, a novel estrogen receptor coactivator with a bifunctional nuclear receptor interacting determinant. Molec. Cell. Biol. 21: 343-353, 2001.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

49229 MW

Observed Molecular Weight

66 kDa

Applications Notes

6

Gene Name Synonym

SRY-box 15

Subcellular Location

Nucleus.

Protein Name

Chromaffin granule amine transporter

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

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