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Anti-PPM1J Antibody Picoband®

Boster Bio Anti-PPM1J Antibody Picoband® catalog # A15780-2. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

This gene encodes the serine/threonine protein phosphatase. The mouse homolog of this gene apparently belongs to the protein phosphatase 2C family of genes. The exact function of this gene is not yet known.

Synonyms

Kelch repeat and BTB domain-containing protein 2; BTB and kelch domain-containing protein 1; KBTBD2; BKLHD1; KIAA1489

Gene Name

PPM1J

Gene ID

333926

UniProt

Q5JR12

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PPM1J recombinant protein (Position: K59-D475) .

Clonality

Polyclonal

Tissue Specificity

Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Applications

WB, ICC, IF, ELISA

Field of Research

Cell Biology

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.

References & Citations

1. Gross, M. B. Personal Communication. Baltimore, Md. 9/30/2014. 2. Kashiwaba, M., Katsura, K., Ohnishi, M., Sasaki, M., Tanaka, H., Nishimune, Y., Kobayashi, T., Tamura, S. A novel protein phosphatase 2C family member (PP2C-zeta) is able to associate with ubiquitin conjugating enzyme 9. FEBS Lett. 538: 197-202, 2003.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

24145 MW

Observed Molecular Weight

55 kDa

Gene Name Synonym

Kelch repeat and BTB domain containing 2

Subcellular Location

Cul3-RING ubiquitin ligase complex.

Protein Name

Transmembrane protein 240

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

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