Anti-PREX1 Antibody Picoband® Fluoro488 Conjugated

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein is a protein that in humans is encoded by the PREX1 gene. The protein encoded by this gene acts as a guanine nucleotide exchange factor for the RHO family of small GTP-binding proteins (RACs) . It has been shown to bind to and activate RAC1 by exchanging bound GDP for free GTP. The encoded protein, which is found mainly in the cytoplasm, is activated by phosphatidylinositol-3,4,5-trisphosphate and the beta-gamma subunits of heterotrimeric G proteins.

Mainly expressed in pachytene spermatocytes of testis and in lymphocyte-rich areas of spleen and lymph nodes. Isoform v1 is expressed in spleen. Isoform v2 is expressed in testis. Also detected in ovary, placenta, pancreas, cardiac fibroblasts. Expressed in B-cells and prostate malignant cells. Isoform v1 and isoform v3 are expressed in epithelial colorectal adenocarcinoma cells. Isoform v2 and isoform v4 are expressed in endothelial cells. Isoform v1, isoform v2, isoform v3 and isoform v4 are expressed in pulmonary artery smooth muscle cells. Isoform v2 and isoform v5 are expressed in microvascular endothelial cells (at protein level) .

Apoptosis, Associated Proteins, Cell Biology, Cell Cycle, Cell Differentiation, Channels, Intracellular, Metabolism, Oxidative Stress, Pathways and Processes, Plasma Membrane, Redox Metabolism, Signal Transduction

Calcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. May play a role in cell growth and apoptosis. Isoform v2 and isoform v5 are involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin.

1. Donald, S., Humby, T., Fyfe, I., Segonds-Pichon, A., Walker, S. A., Andrews, S. R., Coadwell, W. J., Emson, P., Wilkinson, L. S., Welch, H. C. E. P-Rex2 regulates Purkinje cell dendrite morphology and motor coordination. Proc. Nat. Acad. Sci. 105: 4483-4488, 2008. 2. Welch, H. C. E., Coadwell, W. J., Ellson, C. D., Ferguson, G. J., Andrews, S. R., Erdjument-Bromage, H., Tempst, P., Hawkins, P. T., Stephens, L. R. P-Rex1, a PtdIns (3,4,5) P3- and G-beta-gamma-regulated guanine-nucleotide exchange factor for Rac. Cell 108: 809-821, 2002.