Anti-PSME1 Antibody Picoband® Fluoro550 Conjugated

Proteasome activator complex subunit 1 is a protein that in humans is encoded by the PSME1 gene. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Alternative splicing results in multiple transcript variants.

Solute carrier family 12 member 5; Electroneutral potassium-chloride cotransporter 2; K-Cl cotransporter 2; hKCC2; Neuronal K-Cl cotransporter; SLC12A5; KCC2; KIAA1176

Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl- homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl- levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition. Involved in the regulation of dendritic spine formation and maturation.

1. Belich, M. P., Glynne, R. J., Senger, G., Sheer, D., Trowsdale, J. Proteasome components with reciprocal expression to that of the MHC-encoded LMP proteins. Curr. Biol. 4: 769-776, 1994. 2. Honore, B., Leffers, H., Madsen, P., Celis, J. E. Interferon-gamma up-regulates a unique set of proteins in human keratinocytes: molecular cloning and expression of the cDNA encoding the RGD-sequence-containing protein IGUP I5111. Europ. J. Biochem. 218: 421-430, 1993. 3. McCusker, D., Jones, T., Sheer, D., Trowsdale, J. Genetic relationships of the genes encoding the human proteasome beta subunits and the proteasome PA28 complex. Genomics 45: 362-367, 1997.