Anti-PRSS35 Antibody Picoband®

Proteolytic degradation of extracellular matrix components has been suggested to play an essential role for the occurrence of ovulation. The plasminogen activator and matrix metalloproteinase systems, which were previously believed to be crucial for ovulation, are not required in this process. PRSS35, which was upregulated by gonadotropins. PRSS23 was highly expressed in atretic follicles and it was expressed in the ovarian stroma and theca tissues just prior to ovulation. PRSS35 was expressed in the theca layers of developing follicles. It was also highly induced in granulosa cells of preovulatory follicles. PRSS35 was also expressed in the forming and regressing CL. PRSS35 may be involved in ovulation and CL formation and regression, and that PRSS23 may play a role in follicular atresia.

Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.

1. Miyakoshi, K. , Murphy, M. J. , Yeoman, R. R. , Mitra, S. , Dubay, C. J. , & Hennebold, J. D. . (2006) . The identification of novel ovarian proteases through the use of genomic and bioinformatic methodologies. Biology of Reproduction, 75 (6), 823-35. 2. Floyd, Brendan, J. , Wilkerson, Emily, M. , Veling, & Mike, T. , et al. Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function. 3. Clark, H. F. , Gurney, A. L. , Abaya, E. , Baker, K. , Baldwin, D. , & Brush, J. , et al. (2003) . The secreted protein discovery initiative (spdi), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. Genome Research, 13 (10), 2265-2276.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.