Anti-RNASEH1 Antibody Picoband® Fluoro488 Conjugated

Ribonuclease H1 also known as RNase H1 is an enzyme that in humans is encoded by the RNASEH1 gene. This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and plays a key role in DNA replication and repair. Alternate in-frame start codon initiation results in the production of alternate isoforms that are ed to the mitochondria or to the nucleus. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF) . Mutations in this gene have been found in individuals with progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 2. Alternative splicing results in additional coding and non-coding transcript variants. Pseudogenes of this gene have been defined on chromosomes 2 and 17.

Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation (PubMed:17093495, PubMed:19289083, PubMed:23085020) . Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint (PubMed:17093495) . The SKA1 complex is a component of the kinetochore-microtubule interface and ly associates with microtubules as oligomeric assemblies (PubMed:19289083) . The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner (PubMed:17093495, PubMed:19289083) . In the complex, it is required for SKA1 localization (PubMed:19289083) . Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules (PubMed:23085020) .

1. Cerritelli, S. M., Crouch, R. J. Cloning, expression, and mapping of ribonucleases H of human and mouse related to bacterial RNase H1. Genomics 53: 300-307, 1998. 2. Cerritelli, S. M., Frolova, E. G., Feng, C., Grinberg, A., Love, P. E., Crouch, R. J. Failure to produce mitochondrial DNA results in embryonic lethality in Rnaseh1 null mice. Molec. Cell 11: 807-815, 2003. 3. Nowotny, M., Gaidamakov, S. A., Ghirlando, R., Cerritelli, S. M., Crouch, R. J., Yang, W. Structure of human RNase H1 complexed with an RNA/DNA hybrid: insight into HIV reverse transcription. Molec. Cell 28: 264-276, 2007. Note: Erratum: Molec. Cell 28: 513 only, 2007.