Anti-Spt6/SUPT6H Antibody Picoband®

Transcription elongation factor SPT6 is a protein that in humans is encoded by the SUPT6H gene. SUPT6H (suppressor of Ty6 homolog), also known as SPT6, SPT6H, Tat-CT2 (Tat-cotransactivator 2 protein) or emb-5 in C. elegans, is a 1,726 amino acid protein that is highly conserved from yeast to humans. Expressed ubiquitously, SUPT6H localizes to the nucleus and contains one SH2 domain and one S1 domain. SUPT6H participates in both DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole) -mediated transcriptional inhibition as well as the enhancement of transcriptional elongation by the RNA polymerase II (Pol II) . SUPT6H interacts with the nuclear proteins SPT4 and SPT5, which comprise the DSIF (DRB-sensitivity-inducing factor) complex that binds RNA polymerase II, and ly regulates elongation. Via its C-terminus, SUPT6H can also interact with Histone H3. Due to alternative splicing events, three isoforms exist for SUPT6H. This antibody specifically recognizes the 230kd phosphorylated SUPT6H protein.

Mediator of RNA polymerase II transcription subunit 4; Activator-recruited cofactor 36 kDa component; ARC36; Mediator complex subunit 4; TRAP/SMCC/PC2 subunit p36 subunit; Vitamin D3 receptor-interacting protein complex 36 kDa component; DRIP36; MED4; ARC36; DRIP36; VDRIP; HSPC126

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

1. Andrulis, E. D., Werner, J., Nazarian, A., Erdjument-Bromage, H., Tempst, P., Lis, J. T. The RNA processing exosome is linked to elongating RNA polymerase II in Drosophila. Nature 420: 837-841, 2002. 2. Chiang, P.-W., Wang, S., Smithivas, P., Song, W.-J., Ramamoorthy, S., Hillman, J., Puett, S., Van Keuren, M. L., Crombez, E., Kumar, A., Glover, T. W., Miller, D. E., Tsai, C.-H., Blackburn, C. C., Chen, X.-N., Sun, Z., Cheng, J.-F., Korenberg, J. R., Kurnit, D. M. Identification and analysis of the human and murine putative chromatin structure regulator SUPT6H and Supt6h. Genomics 34: 328-333, 1996. 3. Kaplan, C. D., Laprade, L., Winston, F. Transcription elongation factors repress transcription initiation from cryptic sites. Science 301: 1096-1099, 2003.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.