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Anti-Aspartate Aminotransferase/GOT1 Antibody Picoband® (monoclonal, 6B3B4)

Boster Bio Anti-Aspartate Aminotransferase/GOT1 Antibody Picoband® (monoclonal, 6B3B4) catalog # M04085-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Aspartate aminotransferase, cytoplasmic is an enzyme that in humans is encoded by the GOT1 gene. Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

Synonyms

SUMO-activating enzyme subunit 2; Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; UBA2; SAE2, UBLE1B; HRIHFB2115

Gene Name

GOT1

Gene ID

2805

UniProt

P17174

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Aspartate Aminotransferase/GOT1 recombinant protein (Position: S5-Q413) .

Clonality

Monoclonal

Clone

Clone: 6B3B4

Applications

WB, IHC, Flow Cytometry

Field of Research

Cell Adhesion Proteins, Epigenetics and Nuclear Signaling, Integrins, Mediator Complex, Neuroscience, Receptors, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

References & Citations

1. Aitken, D. A., Ferguson-Smith, M. A. Gene dosage evidence for the regional assignment of the GOT-S structural gene locus to 10q24-10q25. Cytogenet. Cell Genet. 22: 468-471, 1978. 2. Creagan, R., Tischfield, J., McMorris, F. A., Chen, S.-H., Hirschi, M., Chen, T.-T., Ricciuti, F., Ruddle, F. H. Assignment of the genes for human peptidase A to chromosome 18 and cytoplasmic glutamic oxaloacetate transaminase to chromosome 10 using somatic-cell hybrids. Cytogenet. Cell Genet. 12: 187-198, 1973. 3. Doonan, S., Barra, D., Bossa, F. Structural and genetic relationships between cytosolic and mitochondrial isoenzymes. Int. J. Biochem. 16: 1193-1199, 1984.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

43 kDa

Applications Notes

6

Gene Name Synonym

Ubiquitin like modifier activating enzyme 2

Subcellular Location

Nucleus; Cytoplasm

Isotype

IgG2b

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

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