Anti-Thymidylate Synthase/TYMS Antibody Picoband® Fluoro647 Conjugated

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy.

1. Chu, J., Dolnick, B. J. Natural antisense (rTS-alpha) RNA induces site-specific cleavage of thymidylate synthase mRNA. Biochim. Biophys. Acta 1587: 183-193, 2002. 2. Hori, T., Ayusawa, D., Shimizu, K., Koyama, H., Seno, T. Assignment of human gene encoding thymidylate synthase to chromosome 18 using interspecific cell hybrids between thymidylate synthase-negative mouse mutant cells and human diploid fibroblasts. Somat. Cell Molec. Genet. 11: 277-283, 1985. 3. Hori, T., Ayusawa, D., Shimizu, K., Koyama, H., Seno, T. Assignment of the human TMS gene, encoding thymidylate synthase, to chromosome 18. (Abstract) Cytogenet. Cell Genet. 40: 654-655, 1985.