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Anti-TRIM16 Antibody Picoband®

Boster Bio Anti-TRIM16 Antibody Picoband® catalog # A09514-5. Tested in ELISA, Flow Cytometry, ICC/IF, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Tripartite motif-containing protein 16 is a protein that in humans is encoded by the TRIM16 gene. The protein encoded by this gene is a tripartite motif (TRIM) family member that contains two B box domains and a coiled-coiled region that are characteristic of the B box zinc finger protein family. While it lacks a RING domain found in other TRIM proteins, the encoded protein can homodimerize or heterodimerize with other TRIM proteins and has E3 ubiquitin ligase activity. This gene is also a tumor suppressor and is involved in secretory autophagy.

Synonyms

Mediator of RNA polymerase II transcription subunit 20; Mediator complex subunit 20; TRF-proximal protein homolog; hTRFP; MED20; TRFP

Gene Name

TRIM16

Gene ID

10626

UniProt

O95361

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human TRIM16 recombinant protein (Position: M1-Q218) .

Clonality

Polyclonal

Tissue Specificity

Pre-B-cells and B-cells but not terminally differentiated plasma cells.

Applications

WB, IHC, ICC/IF, Flow Cytometry, ELISA

Field of Research

Epigenetics and Nuclear Signaling, Mediator Complex, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

References & Citations

1. Beer, H.-D., Munding, C., Dubois, N., Mamie, C., Hohl, D., Werner, S. The estrogen-responsive B box protein: a novel regulator of keratinocyte differentiation. J. Biol. Chem. 277: 20740-20749, 2002. 2. Liu, H.-L. C., Golder-Novoselsky, E., Seto, M. H., Webster, L., McClary, J., Zajchowski, D. A. The novel estrogen-responsive B box protein (EBBP) gene is tamoxifen regulated in cells expressing an estrogen receptor DNA-binding domain mutant. Molec. Endocr. 12: 1733-1748, 1998.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

64 kDa

Observed Molecular Weight

64 kDa

Applications Notes

6

Gene Name Synonym

CD72 molecule

Subcellular Location

Nucleus .

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

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