Anti-SERPINB13 Antibody Picoband®

Serpin B13 is a protein that in humans is encoded by the SERPINB13 gene. The protein encoded by this gene is a member of the serpin family of serine protease inhibitors. The encoded protein inhibits the activity of cathepsin K and is itself transcriptionally repressed by RUNX1. This gene is downregulated in many types of cancer.

Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as calcium-dependent adapter for the BCR (KLHL12) complex, a complex involved in endoplasmic reticulum (ER) -Golgi transport by regulating the size of COPII coats. In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR (KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification. Its role in the heterodimer formed with PDCD6 is however unclear: some evidences show that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium. Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6. Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31.

1. Abts, H. F., Breuhahn, K., Michel, G., Kohrer, K., Esser, P., Ruzicka, T. Analysis of UVB modulated gene expression in human keratinocytes by mRNA differential display polymerase chain reaction. Photochem. Photobiol. 66: 363-367, 1997. 2. Abts, H. F., Welss, T., Mirmohammadsadegh, A., Kohrer, K., Michel, G., Ruzicka, T. Cloning and characterization of hurpin (protease inhibitor 13) : a new skin-specific, UV-repressible serine proteinase inhibitor of the ovalbumin serpin family. J. Molec. Biol. 293: 29-39, 1999. 3. Nakashima, T., Pak, S. C., Silverman, G. A., Spring, P. M., Frederick, M. J., Clayman, G. L. Genomic cloning, mapping, structure and promoter analysis of HEADPIN, a serpin which is down-regulated in head and neck cancer cells. Biochim. Biophys. Acta 1492: 441-446, 2000.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.