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Anti-ERP29 Antibody Picoband®

Boster Bio Anti-ERP29 Antibody Picoband® catalog # A03621-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Endoplasmic reticulum protein 29 (ERp29) is a chaperone protein that in humans is encoded by the ERP29 gene. This gene encodes a protein which localizes to the lumen of the endoplasmic reticulum (ER) . It is a member of the protein disulfide isomerase (PDI) protein family but lacks an active thioredoxin motif, suggesting that this protein does not function as a disulfide isomerase. The canonical protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms.

Synonyms

Protocadherin-15; PCDH15; USH1F

Gene Name

ERP29

Gene ID

10961

UniProt

P30040

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human ERP29 recombinant protein (Position: L33-K196) .

Clonality

Polyclonal

Tissue Specificity

Expressed in brain, lung, kidney, spleen and testis. Found also in the inner and outer synaptic layers, and the nerve fiber layer in adult and fetal retinas. Found in the supporting cells, outer sulcus cells and spiral ganglion of fetal cochlea. Expressed in cytotoxic tumor-derived T- and NK-cell lines as well as biopsies of nasal NK/T-cell lymphomas. Not detected in normal or in vitro activated peripheral blood cells, CD4 or CD8 lymphocytes or NK cells. Isoform 3 is expressed in brain, heart, cerebellum and kidney. CD1 isoforms, such as isoform 1, have a limited pattern of expression and is detected in testis, retina and cochlea. CD2 isoforms, such as isoforms 4 and 5, are expressed in heart, kidney, thymus, spleen, testis, retina and cochlea. CD3 isoforms, such as isoform 6, are widely expressed.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Cancer Susceptibility, Epigenetics and Nuclear Signaling, Transcription, Tumor Suppressors

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Calcium-dependent cell-adhesion protein. Essential for maintenance of normal retinal and cochlear function.

References & Citations

1. Demmer, J., Zhou, C., Hubbard, M. J. Molecular cloning of ERp29, a novel and widely expressed resident of the endoplasmic reticulum. FEBS Lett. 402: 145-150, 1997. 2. Ferrari, D. M., Nguyen Van, P., Kratzin, H. D., Soling, H.-D. ERp28, a human endoplasmic-reticulum-lumenal protein, is a member of the protein disulfide isomerase family but lacks a CXXC thioredoxin-box motif. Europ. J. Biochem. 255: 570-579, 1998.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

28461 MW

Observed Molecular Weight

29 kDa

Applications Notes

6

Gene Name Synonym

Protocadherin related 15

Subcellular Location

Cell membrane. Single-pass type I. membrane protein. Secreted.

Protein Name

Breast cancer metastasis-suppressor 1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

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