What sizes are available for Anti-PDCD4 Antibody Picoband® Fluoro647 Conjugated?

Anti-PDCD4 Antibody Picoband® Fluoro647 Conjugated is available in the following size(s): 100 µg/Vial.

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

Anti-PDCD4 Antibody Picoband® Fluoro647 Conjugated

Q: What is the catalog number for Anti-PDCD4 Antibody Picoband® Fluoro647 Conjugated?

The catalog number for Anti-PDCD4 Antibody Picoband® Fluoro647 Conjugated is A01105-3-Fluoro647.

Q: Is Anti-PDCD4 Antibody Picoband® Fluoro647 Conjugated in stock?

Anti-PDCD4 Antibody Picoband® Fluoro647 Conjugated is currently made to order / not held in immediate stock. Contact us for lead time.

Product Specifications

Background

Programmed cell death protein 4 (PDCD4) is a protein that in humans is encoded by the PDCD4 gene. It is mapped to 10q25.2. This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. It also encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined.

Synonyms

Programmed cell death protein 4; Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a; PDCD4; H731

Gene Name

PDCD4

Gene ID

27250

UniProt

Q53EL6

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region human PDCD4, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma. .

Applications

Flow Cytometry

Field of Research

Apoptosis, Cancer, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Cell Death, Epigenetics and Nuclear Signaling, Invasion/Microenvironment, Nucleus, Transcription

Purification

Immunogen affinity purified.

Form

Liquid

Function

Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA (By similarity) . .

References & Citations

1. Azzoni, L; Zatsepina, O; Abebe, B; Bennett, IM; Kanakaraj, P; Perussia, B (1998) . Differential transcriptional regulation of CD161 and a novel gene, 197/15a, by IL-2, IL-15, and IL-12 in NK and T cells. Journal of immunology 161 (7) : 3493–500. 2. Kang MJ, Ahn HS, Lee JY, Matsuhashi S, Park WY (2002) . Up-regulation of PDCD4 in senescent human diploid fibroblasts. Biochemical and Biophysical Research Communications. 293 (1) : 617–21.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Calculated Molecular Weight

51735 MW

Applications Notes

Gene Name Synonym

Programmed cell death protein 4

Subcellular Location

Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.