Anti-KLF10 Antibody Picoband®

Krueppel-like factor 10 is a protein that in humans is encoded by the KLF10 gene. This gene encodes a member of a family of proteins that feature C2H2-type zinc finger domains. The encoded protein is a transcriptional repressor that acts as an effector of transforming growth factor beta signaling. Activity of this protein may inhibit the growth of cancers, particularly pancreatic cancer. Alternative splicing results in multiple transcript variants.

Vacuolar protein sorting-associated protein 4B; Cell migration-inducing gene 1 protein; Suppressor of K (+) transport growth defect 1; Protein SKD1; VPS4B; SKD1; VPS42; MIG1

Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses) . VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan.

1. Blok, L. J., Grossmann, M. E., Perry, J. E., Tindall, D. J. Characterization of an early growth response gene which encodes a zinc finger transcription factor potentially involved in cell cycle regulation. Molec. Endocr. 9: 1610-1620, 1995. 2. Fautsch, M. P., Vrabel, A., Rickard, D., Subramaniam, M., Spelsberg, T. C., Wieben, E. D. Characterization of the mouse TGF-beta-inducible early gene (TIEG) : conservation of exon and transcriptional regulatory sequences with evidence of additional transcripts. Mammalian Genome 9: 838-842, 1998. 3. Fautsch, M. P., Vrabel, A., Subramaniam, M., Hefferen, T. E., Spelsberg, T. C., Wieben, E. D. TGF-beta-inducible early gene (TIEG) also codes for early growth response alpha (EGR-alpha) : evidence of multiple transcripts from alternate promoters. Genomics 51: 408-416, 1998.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.