Anti-MDH2 Antibody Picoband® (monoclonal, 5D8C1)

Malate dehydrogenase, mitochondrial also known as malate dehydrogenase 2 is an enzyme that in humans is encoded by the MDH2 gene. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene.

E.coli-derived human MDH2 recombinant protein (Position: A9-L223) . Human MDH2 shares 97.7% and 98.1% amino acid (aa) sequence identity with mouse and rat MDH2, respectively.

Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. Its F-actin depolymerization activity is regulated by association with CSPR3. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Required for muscle maintenance. May play a role during the exchange of alpha-actin forms during the early postnatal remodeling of the sarcomere.

1. Ait-El-Mkadem, S., Dayem-Quere, M., Gusic, M., Chaussenot, A., Bannwarth, S., Francois, B., Genin, E. C., Fragaki, K., Volker-Touw, C. L. M., Vasnier, C., Serre, V., van Gassen, K. L. I., and 24 others. Mutations in MDH2, encoding a Krebs cycle enzyme, cause early-onset severe encephalopathy. Am. J. Hum. Genet. 100: 151-159, 2017. 2. Benn, P., Chern, C. J., Bruns, G., Craig, I. W., Croce, C. M. Assignment of the genes for human beta-glucuronidase and mitochondrial malate dehydrogenase to the region pter-q22 of chromosome 7. Cytogenet. Cell Genet. 19: 273-280, 1977. 3. Blake, N. M. Malate dehydrogenase types in the Asian-Pacific area, and a description of new phenotypes. Hum. Genet. 43: 69-80, 1978.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.