Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

Anti-MPPB/PMPCB Antibody Picoband® (monoclonal, 9F13E4) Fluoro647 Conjugated

Q: What is the catalog number for Anti-MPPB/PMPCB Antibody Picoband® (monoclonal, 9F13E4) Fluoro647 Conjugated?

The catalog number for Anti-MPPB/PMPCB Antibody Picoband® (monoclonal, 9F13E4) Fluoro647 Conjugated is M11793-Fluoro647.

Product Specifications

Background

Mitochondrial-processing peptidase subunit beta is an enzyme that in humans is encoded by the PMPCB gene. This gene is a member of the peptidase M16 family and encodes a protein with a zinc-binding motif. This protein is located in the mitochondrial matrix and catalyzes the cleavage of the leader peptides of precursor proteins newly imported into the mitochondria, though it only functions as part of a heterodimeric complex.

Synonyms

Gene Name

PMPCB

Gene ID

9512

UniProt

O75439

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human MPPB/PMPCB recombinant protein (Position: E23-Q479) .

Clonality

Monoclonal

Clone

Clone: 9F13E4

Tissue Specificity

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Applications

Flow Cytometry

Field of Research

Chaperones, Protein Trafficking, Signal Transduction

Purification

Immunogen affinity purified.

Form

Liquid

Function

Mitochondrial membrane ATP synthase (F (1) F (0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F (1) - containing the extramembraneous catalytic core, and F (0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F (1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F (0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha (3) beta (3) subcomplex and subunit a/ATP6 static relative to the rotary elements.

References & Citations

1. Fields, S., Song, O. A novel genetic system to detect protein-protein interactions. (Letter) Nature 340: 245-246, 1989. 2. Gordon, D. M., Shi, Q., Dancis, A., Pain, D. Maturation of frataxin within mammalian and yeast mitochondria: one-step processing by matrix processing peptidase. Hum. Molec. Genet. 8: 2255-2262, 1999. 3. Gross, M. B. Personal Communication. Baltimore, Md. 5/3/2018.