Anti-HNF-4-alpha Antibody Picoband® (monoclonal, 6C8E9)

Hepatocyte nuclear factor 4 alpha (HNF4A), also known as NR2A1, is a nuclear receptor that in humans is encoded by the HNF4A gene. It is mapped to 20q13.12. HNF4A is a nuclear transcription factor that binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene plays a role in development of the liver, kidney, and intestines. HNF4A is required for the PXR and CAR-mediated transcriptional activation of CYP3A4. This gene also plays a pivotal role in the expression and synthesis of SHBG, an important glycoprotein made primarily in the liver, which in addition to lowering insulin-resistance also serves in reducing levels of free Oestrogen as-well as prolonging the half-life of Testosterone.

AHUS2 antibody; Antigen defined by monoclonal antibody; TRA 2 10 antibody; Antigen identified by monoclonal antibody; TRA 2 10 antibody; CD46 antibody; CD46 antigen antibody; CD46 antigen complement regulatory protein antibody; CD46 molecule antibody; CD46 molecule complement regulatory protein antibody; Complement membrane cofactor protein antibody; MCP antibody; MCP_HUMAN antibody; Measles virus receptor antibody; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen) antibody; Membrane cofactor protein antibody; MGC26544 antibody; MIC10 antibody; TLX antibody; TRA2.10 antibody; Trophoblast leucocyte common antigen antibody; Trophoblast leukocyte common antigen antibody; Trophoblast lymphocyte cross reactive antigen antibody

E.coli-derived human HNF-4-alpha recombinant protein (Position: Q164-I474) . Human HNF-4-alpha shares 95% and 96% amino acid (aa) sequence identity with mouse and rat HNF-4-alpha, respectively.

Cell Biology, Cell Cycle, Immunology, Innate Immunity, Kinases/Phosphatases, Lipid Signaling, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction, Signaling Pathway, TLR Signaling

Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity.

1. Chartier FL, Bossu JP, Laudet V, Fruchart JC, Laine B (September 1994) . Cloning and sequencing of cDNAs encoding the human hepatocyte nuclear factor 4 indicate the presence of two isoforms in human liver. Gene 147 (2) : 269–72. 2. Argyrokastritis A, Kamakari S, Kapsetaki M, Kritis A, Talianidis I, Moschonas NK (February 1997) . Human hepatocyte nuclear factor-4 (hHNF-4) gene maps to 20q12-q13.1 between PLCG1 and D20S17. Hum. Genet. 99 (2) : 233–6. 3. Tirona RG, Lee W, Leake BF, Lan LB, Cline CB, Lamba V, Parviz F, Duncan SA, Inoue Y, Gonzalez FJ, Schuetz EG, Kim RB (February 2003) . The orphan nuclear receptor HNF4alpha determines PXR- and CAR-mediated xenobiotic induction of CYP3A4. Nat. Med. 9 (2) : 220–4.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.