Anti-Desmoglein 3/PVA/DSG3 Antibody Picoband®

Desmoglein3 is also known as PVA (Pemphigus Vulgaris Antigen) . Pemphigus vulgaris (PV) and pemphigus foliaceus (PF) are autoimmune diseases of the skin which have as target antigens 2 different members of the desmoglein subfamily of the desmosomal cadherins: pemphigus vulgaris antigen (PVA, or desmoglein-3) in the case of PV and desmoglein I in the case of PF. The DSG3 gene, like the genes for DSG1 and DSG2, is located on chromosome 18. The human DSG3 gene consists of 15 exons and spans more than 23 kb. DSG3 and DSG1 are the autoantigens of pemphigus vulgaris and pemphigus foliaceus, respectively. DSG3 and DSG1 are the target antigens in PNP, and IgG autoantibodies against DSG3 in PNP sera play a pathogenic role in the loss of keratinocyte adhesion and blistering.

Mainly expressed in pachytene spermatocytes of testis and in lymphocyte-rich areas of spleen and lymph nodes. Isoform v1 is expressed in spleen. Isoform v2 is expressed in testis. Also detected in ovary, placenta, pancreas, cardiac fibroblasts. Expressed in B-cells and prostate malignant cells. Isoform v1 and isoform v3 are expressed in epithelial colorectal adenocarcinoma cells. Isoform v2 and isoform v4 are expressed in endothelial cells. Isoform v1, isoform v2, isoform v3 and isoform v4 are expressed in pulmonary artery smooth muscle cells. Isoform v2 and isoform v5 are expressed in microvascular endothelial cells (at protein level) .

Apoptosis, Associated Proteins, Cell Biology, Cell Cycle, Cell Differentiation, Channels, Intracellular, Metabolism, Oxidative Stress, Pathways and Processes, Plasma Membrane, Redox Metabolism, Signal Transduction

Calcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. May play a role in cell growth and apoptosis. Isoform v2 and isoform v5 are involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin.

1. Amagai, M., Klaus-Kovtun, V., Stanley, J. R.Autoantibodies against a novel epithelial cadherin in pemphigus vulgaris, a disease of cell adhesion.Cell 67: 869-877, 1991. 2. Arnemann, J., Spurr, N. K., Buxton, R. S.The human gene (DSG3) coding for the pemphigus vulgaris antigen is, like the genes coding for the other two known desmogleins, assigned to chromosome 18.Hum. Genet. 89: 347-350, 1992. 3. Silos, S. A., Tamai, K., Li, K., Kivirikko, S., Kouba, D., Christiano, A. M., Uitto, J.Cloning of the gene for human pemphigus vulgaris antigen (desmoglein 3), a desmosomal cadherin.J. Biol. Chem. 271: 17504-17511, 1996.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.