Anti-PU.1/SPI1 Antibody Picoband® Fluoro488 Conjugated

Transcription factor PU.1 is a protein that in humans is encoded by the SPI1 gene. This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.

CD40 ligand; CD40-L; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD154; CD40LG; CD40L; TNFSF5; TRAP

Adaptive Immunity, Atherosclerosis, Cardiovascular, Cytokines, Immunoglobulins, Immunology, Innate Immunity, Platelets, T Cells, TNF Superfamily, Vascular Inflammation

Cytokine that binds to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. Induces tyrosine phosphorylation of isoform 3 of CD28. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching (By similarity) . Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and a matrix metalloproteinases (MMP) inhibitor-sensitive pathway.

1. Entrez Gene: SPI1 spleen focus forming virus (SFFV) proviral integration oncogene spi1. 2. Hallier M, Lerga A, Barnache S, Tavitian A, Moreau-Gachelin F (February 1998) . The transcription factor Spi-1/PU.1 interacts with the potential splicing factor TLS. J. Biol. Chem. 273 (9) : 4838–42. 3. Ray D, Culine S, Tavitain A, Moreau-Gachelin F (July 1990) . The human homologue of the putative proto-oncogene Spi-1: characterization and expression in tumors. Oncogene 5 (5) : 663–8.