Anti-NFIB/NF1B2 Antibody Picoband® (monoclonal, 4D6E4)

Nuclear factor 1 B-type is a protein that in humans is encoded by the NFIB gene. The NFIB gene is a part of the NFI gene complex that includes three other genes (NFIA, NFIC and NFIX) . The NFIB gene is a protein coding gene that also serves as a transcription factor. This gene is essential in embryonic development and it works together with its gene complex to initiate tissue differentiation in the fetus. Through knockout experiments, researchers found that mice without the NFIB gene have severely underdeveloped lungs. This mutation does not seem to cause spontaneous abortions because in utero the fetus does not use its lungs for respiration. However, this becomes lethal once the fetus is born and has to take its first breath. It is thought that NFIB plays a role in down regulating the transcription factors TGF-β1 and Shh in normal gestation because they remained high in knockout experiments. The absence of NFIB also leads to insufficient amounts of surfactant being produced which is one reason why the mice cannot breathe once it is born. The knockout experiments demonstrated that NFIB has a significant role in fore-brain development. NFIB is typically found in pontine nuclei of the CNS, the cerebral cortex and the white matter of the brain and without NFIB these areas are dramatically affected.

Adhesion molecule antibody; CD31 antibody; CD31 antigen antibody; CD31 EndoCAM antibody; Endocam antibody; FLJ34100 antibody; FLJ58394 antibody; GPIIA antibody; GPIIA' antibody; PECA1 antibody; PECA1_HUMAN antibody; Pecam 1 antibody; PECAM 1 CD31 EndoCAM antibody; PECAM antibody; PECAM-1 antibody; Pecam1 antibody; Platelet endothelial cell adhesion molecule antibody; Platelet/endothelial cell adhesion molecule 1 antibody; Platelet/endothelial cell adhesion molecule antibody

Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Expressed in human umbilical vein endothelial cells (HUVECs) (at protein level) . Expressed on neutrophils (at protein level) . Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level) .

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis. Does not protect against apoptosis.

1. Gründer, Albert; Ebel, Thorsten T.; Mallo, Moisés; Schwarzkopf, Georg; Shimizu, Takehiko; Sippel, Albrecht E.; Schrewe, Heinrich (2002-03-01) . Nuclear factor I-B (Nfib) deficient mice have severe lung hypoplasia. Mechanisms of Development. 112 (1–2) : 69–77. 2. Steele-Perkins, George; Plachez, Céline; Butz, Kenneth G.; Yang, Guanhu; Bachurski, Cindy J.; Kinsman, Stephen L.; Litwack, E. David; Richards, Linda J.; Gronostajski, Richard M. (2005-01-15) . The Transcription Factor Gene Nfib Is Essential for both Lung Maturation and Brain Development. Molecular and Cellular Biology. 25 (2) : 685–698.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.