Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Anti-splicing factor 1 Antibody Picoband® (monoclonal, 2F5D10) Fluoro594 Conjugated

Product Specifications

Background

Splicing factor 1 also known as zinc finger protein 162 (ZFM162) is a protein that in humans is encoded by the SF1 gene. This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.

Synonyms

ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial; CLPX

Gene Name

SF1

Gene ID

7536

UniProt

Q15637

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human splicing factor 1 recombinant protein (Position: R160-Q266) .

Clonality

Monoclonal

Clone

Clone: 2F5D10

Tissue Specificity

Higher expression in skeletal muscle and heart and to a lesser extent in liver, brain, placenta, lung, kidney and pancreas.

Applications

Flow Cytometry

Field of Research

Adapters, Cytokines, Immunology, Innate Immunity, Interleukins, Macrophage/Inflammation, Signal Transduction, Transmembrane

Purification

Immunogen affinity purified.

Form

Liquid

Function

ATP-dependent specificity component of the Clp protease complex. Hydrolyzes ATP (PubMed:28874591) . Targets specific substrates for degradation by the Clp complex (PubMed:11923310, PubMed:22710082) . Can perform chaperone functions in the absence of CLPP. Enhances the DNA-binding activity of TFAM and is required for maintaining a normal mitochondrial nucleoid structure (PubMed:22841477) . ATP-dependent unfoldase that stimulates the incorporation of the pyridoxal phosphate cofactor into 5-aminolevulinate synthase, thereby activating 5-aminolevulinate (ALA) synthesis, the first step in heme biosynthesis (PubMed:28874591) . Important for efficient erythropoiesis through upregulation of heme biosynthesis (PubMed:25957689, PubMed:28874591) .

References & Citations

1. Toda, T., Iida, A., Miwa, T., Nakamura, Y., Imai, T. Isolation and characterization of a novel gene encoding nuclear protein at a locus (D11S636) tightly linked to multiple endocrine neoplasia type 1 (MEN1) . Hum. Molec. Genet. 3: 465-470, 1994. 2. Caslini, C., Spinelli, O., Cazzaniga, G., Golay, J., De Gioia, L., Pedretti, A., Breviario, F., Amaru, R., Barbui, T., Biondi, A., Introna, M., Rambaldi, A. Identification of two novel isoforms of the ZNF162 gene: a growing family of signal transduction and activator of RNA proteins. Genomics 42: 268-277, 1997.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Applications Notes

6

Gene Name Synonym

Caseinolytic mitochondrial matrix peptidase chaperone subunit

Subcellular Location

Mitochondrion; mitochondrion nucleoid

Isotype

Mouse IgG2a

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

Human UPP1 knockout cell line
ABC-KH16574 1 Vial

Human UPP1 knockout cell line

Ask
View Details
ND3 Rabbit Polyclonal Antibody
TA378770 100 µL

ND3 Rabbit Polyclonal Antibody

Ask
View Details
LINC02872 Rabbit Polyclonal Antibody
TA334913 100 µL

LINC02872 Rabbit Polyclonal Antibody

Ask
View Details
Handheld Mini Centrifuge
E0127 1 Unit

Handheld Mini Centrifuge

Ask
View Details