Anti-CARM1 Antibody Picoband®

CARM1 (coactivator-associated arginine methyltransferase 1), also known as PRMT4, is an enzyme encoded by the CARM1 gene found in human beings, as well as many other mammals. This gene is mapped to 19p13.2. CARM1 is a regulator of cyclin E1 and DHFR mRNA expression. Its main function includes catalyzing the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. CARM1 is a secondary coactivator through its association with p160 family (SRC-1, GRIP1, AIB) of coactivators. It is responsible for moving cells toward the inner cell mass in developing blastocysts. This gene also plays an important role in androgen receptors and may play a role in prostate cancer progression.

Predominantly expressed in the liver (PubMed:11099417, PubMed:11452359) . Low expression levels in the small intestine and colon (PubMed:11099417) . Very low levels in other tissues, including brain, heart and spleen (PubMed:11452359) .

Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Cholesterol Metabolism, Heart Disease, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Pathways and Processes, Signal Transduction

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

1. Chen D, Ma H, Hong H, Koh SS, Huang SM, Schurter BT, Aswad DW, Stallcup MR (1999) . Regulation of transcription by a protein methyltransferase. Science 284 (5423) : 2174–7. 2. El Messaoudi, S. E., Fabbrizio, E., Rodriguez, C., Chuchana, P., Fauquier, L., Cheng, D., Theillet, C., Vandel, L., Bedford, M. T., Sardet, C. Coactivator-associated arginine methyltransferase 1 (CARM1) is a positive regulator of the cyclin E1 gene. Proc. Nat. Acad. Sci. 103: 13351-13356, 2006. 3. Torres-Padilla ME, Parfitt DE, Kouzarides T, Zernicka-Goetz M (2007) . Histone arginine methylation regulates pluripotency in the early mouse embryo. Nature445 (7124) : 214–8.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.