Anti-GNB3 Antibody Picoband®

Guanine nucleotide-binding protein G (I) /G (S) /G (T) subunit beta-3 is a protein that in humans is encoded by the GNB3 gene. Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit which belongs to the WD repeat G protein beta family. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. A single-nucleotide polymorphism (C825T) in this gene is associated with essential hypertension and obesity. This polymorphism is also associated with the occurrence of the splice variant GNB3-s, which appears to have increased activity. GNB3-s is an example of alternative splicing caused by a nucleotide change outside of the splice donor and acceptor sites. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.

Specifically expressed in endothelial cells. Detected in monocytes, placenta, lung, liver, kidney, skeletal muscle, pancreas, spleen, prostate, small intestine and colon.

Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes (By similarity) . .

1. Danciger, M., Chakraborti, A., Farber, D. B., Kozak, C. A. Localization of the gene for a third G protein beta-subunit to mouse chromosome 6 near Raf-1. Genomics 12: 688-692, 1992. 2. Dishy, V., Gupta, S., Landau, R., Xie, H.-G., Kim, R. B., Smiley, R. M., Byrne, D. W., Wood, A. J. J., Stein, C. M. G-protein beta-3 subunit 825 C/T polymorphism is associated with weight gain during pregnancy. Pharmacogenetics 13: 241-242, 2003. 3. Feldman, R. D., Hegele, R. A. G-protein polymorphisms and maternal/neonatal metabolism: still a weight for the answer. (Commentary) Lancet 355: 1201-1202, 2000.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytosol . Cytoplasmic vesicle . Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta (By similarity) . .