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Anti-WDR77 Antibody Picoband®

Boster Bio Anti-WDR77 Antibody Picoband® catalog # A04894-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Methylosome protein 50 is a protein that in humans is encoded by the WDR77 gene. The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene.

Synonyms

Vesicular acetylcholine transporter; VAChT; Solute carrier family 18 member 3; SLC18A3; VACHT

Gene Name

WDR77

Gene ID

79084

UniProt

Q9BQA1

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human WDR77 recombinant protein (Position: A13-A295) .

Clonality

Polyclonal

Tissue Specificity

Peripheral and central cholinergic nervous systems. .

Applications

WB, ELISA

Field of Research

Neuroscience, Neurotransmitter

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Involved in acetylcholine transport into synaptic vesicles. .

References & Citations

1. Friesen, W. J., Wyce, A., Paushkin, S., Abel, L., Rappsilbers, J., Mann, M., Dreyfuss, G. A novel WD repeat protein component of the methylosome binds Sm proteins. J. Biol. Chem. 277: 8243-8247, 2002. 2. Furuno, K., Masatsugu, T., Sonoda, M., Sasazuki, T., Yamamoto, K. Association of Polycomb group SUZ12 with WD-repeat protein MEP50 that binds to histone H2A selectively in vitro. Biochem. Biophys. Res. Commun. 345: 1051-1058, 2006. 3. Gross, M. B. Personal Communication. Baltimore, Md. 3/2/2018.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

56903 MW

Observed Molecular Weight

42 kDa

Gene Name Synonym

Vesicular acetylcholine transporter

Subcellular Location

Membrane ; Multi-pass membrane protein .

Protein Name

Vesicular acetylcholine transporter

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

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