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Anti-NOL10 Antibody Picoband® Fluoro647 Conjugated

Product Specifications

Background

By immunoprecipitation of human AATF we discovered that AATF forms a salt-stable protein complex together with neuroguidin (NGDN) and NOL10, and demonstrate that the AATF-NGDN-NOL10 (ANN) complex functions in ribosome biogenesis. All three ANN complex members localize to nucleoli and display a mutual dependence with respect to protein stability. Mapping of protein-protein interaction domains revealed the importance of both the evolutionary conserved WD40 repeats in NOL10 and the UTP3/SAS10 domain in NGDN for complex formation.

Synonyms

RNA-binding protein 47; RNA-binding motif protein 47; RBM47

Gene Name

NOL10

Gene ID

79954

UniProt

Q9BSC4

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human NOL10 recombinant protein (Position: Y73-D497) .

Clonality

Polyclonal

Tissue Specificity

Abundantly expressed in tonsil, lymph node, and trachea; strong expression in prostate; lower expression in thyroid, stomach, and colon. .

Applications

Flow Cytometry

Field of Research

Chromatin Binding Proteins, DNA/RNA Binding, Epigenetics and Nuclear Signaling

Purification

Immunogen affinity purified.

Form

Liquid

Function

Can bind to the surface of B-lymphoma cells, but not T- lymphoma cells, consistent with a function as a secreted mediator acting upon B-cells.

References & Citations

1. Hartz, P. A. Personal Communication. Baltimore, Md. 1/20/2015. 2. Wada, K., Sato, M., Araki, N., Kumeta, M., Hirai, Y., Takeyasu, K., Furukawa, K., Horigome, T. Dynamics of WD-repeat containing proteins in SSU processome components. Biochem. Cell Biol. 92: 191-199, 2014.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Calculated Molecular Weight

64099 MW

Applications Notes

6

Gene Name Synonym

RNA-binding protein 47

Subcellular Location

Nucleus .

Protein Name

Follicular dendritic cell secreted peptide

Isotype

Rabbit IgG

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

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