Anti-GCC1 Antibody Picoband® Fluoro647 Conjugated
Product Specifications
Background
GRIP and coiled-coil domain-containing protein 1 is a protein that in humans is encoded by the GCC1 gene. The protein encoded by this gene is a peripheral membrane protein. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. It may play a role in the organization of trans-Golgi network subcompartment involved with membrane transport.
Synonyms
Negative elongation factor B; NELF-B; Cofactor of BRCA1; NELFB; COBRA1; KIAA1182
Gene Name
GCC1
Gene ID
79571
UniProt
Q96CN9
Host
Rabbit
Reactivity
Human, Mouse, Rat
Cross Reactivity
No cross-reactivity with other proteins.
Immunogen
E.coli-derived human GCC1 recombinant protein (Position: E22-L763) .
Clonality
Polyclonal
Tissue Specificity
Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.
Applications
Flow Cytometry
Field of Research
Epigenetics and Nuclear Signaling, Transcription
Purification
Immunogen affinity purified.
Form
Liquid
Function
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Binds RNA which may help to stabilize the NELF complex on nucleic acid. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. May be able to induce chromatin unfolding.
References & Citations
1. Kjer-Nielsen, L., Teasdale, R. D., van Vliet, C., Gleeson, P. A. A novel Golgi-localisation domain shared by a class of coiled-coil peripheral membrane proteins. Curr. Biol. 9: 385-388, 1999. 2. Luke, M. R., Kjer-Nielsen, L., Brown, D. L., Stow, J. L., Gleeson, P. A. GRIP domain-mediated targeting of two new coiled-coil proteins, GCC88 and GCC185, to subcompartments of the trans-Golgi network. J. Biol. Chem. 278: 4216-4226, 2003. 3. Wong, M., Munro, S. The specificity of vesicle traffic to the Golgi is encoded in the golgin coiled-coil proteins. Science 346: 1256898, 2014. Note: Electronic Article.
Storage Conditions
At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Applications Notes
6
Gene Name Synonym
Negative elongation factor complex member B
Subcellular Location
Nucleus.
Isotype
Rabbit IgG
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
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