Anti-DLST Antibody Picoband®

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial is an enzyme that in humans is encoded by the DLST gene. This gene encodes a mitochondrial protein that belongs to the 2-oxoacid dehydrogenase family. This protein is one of the three components (the E2 component) of the 2-oxoglutarate dehydrogenase complex that catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO (2) . Alternatively spliced transcript variants have been found for this gene.

Protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. Processes preferentially poly-SUMO2 and poly-SUMO3 chains, but does not efficiently process SUMO1, SUMO2 and SUMO3 precursors. Deconjugates SUMO1 from RXRA, leading to transcriptional activation. Involved in chromosome alignment and spindle assembly, by regulating the kinetochore CENPH-CENPI-CENPK complex. Desumoylates PML and CENPI, protecting them from degradation by the ubiquitin ligase RNF4, which targets polysumoylated proteins for proteasomal degradation. Desumoylates also RPA1, thus preventing recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination.

1. Ali, G., Wasco, W., Cai, X., Szabo, P., Sheu, K.-F. R., Cooper, A. J. L., Gaston, S. M., Gusella, J. F., Tanzi, R. E., Blass, J. P. Isolation, characterization, and mapping of gene encoding dihydrolipoyl succinyltransferase (E2k) of human alpha-ketoglutarate dehydrogenase complex. Somat. Cell Molec. Genet. 20: 99-105, 1994. 2. Nakano, K., Matuda, S., Sakamoto, T., Takase, C., Nakagawa, S., Ohta, S., Ariyama, T., Inazawa, J., Abe, T., Miyata, T. Human dihydrolipoamide succinyltransferase: cDNA cloning and localization on chromosome 14q24.2-q24.3. Biochim. Biophys. Acta 1216: 360-368, 1993. 3. Nakano, K., Takase, C., Sakamoto, T., Ohta, S., Nakagawa, S., Ariyama, T., Inazawa, J., Abe, T., Matuda, S. An unspliced cDNA for human dihydrolipoamide succinyltransferase: characterization and mapping of the gene to chromosome 14q24.2-q24.3. Biochem. Biophys. Res. Commun. 196: 527-533, 1993.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.