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Anti-ACF1/BAZ1A Antibody Picoband®

Boster Bio Anti-ACF1/BAZ1A Antibody Picoband® catalog # A04090-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Bromodomain adjacent to zinc finger domain protein 1A is a protein that in humans is encoded by the BAZ1A gene. The BAZ1A gene encodes the accessory subunit of the ATP-dependent chromatin assembly factor (ACF), a member of the ISWI ('imitation switch') family of chromatin remodeling complexes.

Synonyms

Muscarinic acetylcholine receptor M1; CHRM1

Gene Name

BAZ1A

Gene ID

11177

UniProt

Q9NRL2

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human ACF1/BAZ1A recombinant protein (Position: E1033-H1536) .

Clonality

Polyclonal

Tissue Specificity

Sperm. Mainly localized in the tail and in the postacrosomal region but is also found in the midpiece and basal region in a small percentage of sperm cells. Reduced levels found in the sperms of asthenozoospermia and leukocytospermia patients (at protein level) . Spleen, lymph nodes, appendix, and fetal liver. Expressed in lymphocytes, T-cells and B-cells but not in natural killer cells, monocytes or granulocytes.

Applications

WB, ICC, IF, Flow Cytometry, ELISA

Field of Research

Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

References & Citations

1. Blosser, T. R., Yang, J. G., Stone, M. D., Narlikar, G. J., Zhuang, X. Dynamics of nucleosome remodelling by individual ACF complexes. Nature 462: 1022-1027, 2009. 2. Bochar, D. A., Savard, J., Wang, W., Lafleur, D. W., Moore, P., Cote, J., Shiekhattar, R. A family of chromatin remodeling factors related to Williams syndrome transcription factor. Proc. Nat. Acad. Sci. 97: 1038-1043, 2000. 3. Collins, N., Poot, R. A., Kukimoto, I., Garcia-Jimenez, C., Dellaire, G., Varga-Weisz, P. D. An ACF1-ISWI chromatin-remodeling complex is required for DNA replication through heterochromatin. Nature Genet. 32: 627-632, 2002.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

211344 MW

Observed Molecular Weight

203 kDa

Applications Notes

6

Gene Name Synonym

Cholinergic receptor muscarinic 1

Subcellular Location

Cell membrane. Multi-pass membrane protein. Postsynaptic cell membrane. Multi-pass membrane protein.

Protein Name

Chromodomain-helicase-DNA-binding protein 2

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

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