Anti-DBC-1/CCAR2 Antibody Picoband®

Deleted in bladder cancer protein 1 is a protein that in humans is encoded by the DBC1 gene. Cell cycle and apoptosis regulator protein 2 (CCAR2), previously known as DBC1/KIAA1967, regulates diverse cellular functions including transcription, mRNA splicing, metabolism, and circadian cycle. CCAR2 is a critical regulator of cell death or survival following cellular stresses. CCAR2 is a widely expressed protein involved in the regulation of a variety of transcriptional complexes. High expression of CCAR2 correlates with poor outcomes in many human tumor types such as squamous cell carcinoma (SCC) .

Vacuolar protein sorting-associated protein 4B; Cell migration-inducing gene 1 protein; Suppressor of K (+) transport growth defect 1; Protein SKD1; VPS4B; SKD1; VPS42; MIG1

Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses) . VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan.

1. Anantharaman, V., Aravind, L. Analysis of DBC1 and its homologs suggests a potential mechanism for regulation of sirtuin domain deacetylases by NAD metabolites. Cell Cycle 7: 1467-1472, 2008. 2. Close, P., East, P., Dirac-Svejstrup, A. B., Hartmann, H., Heron, M., Maslen, S., Chariot, A., Soding, J., Skehel, M., Svejstrup, J. Q. DBIRD complex integrates alternative mRNA splicing with RNA polymerase II transcript elongation. Nature 484: 386-389, 2012. 3. Hamaguchi, M., Meth, J. L., von Klitzing, C., Wei, W., Esposito, D., Rodgers, L., Walsh, T., Welcsh, P., King, M.-C., Wigler, M. H. DBC2, a candidate for a tumor suppressor gene involved in breast cancer. Proc. Nat. Acad. Sci. 99: 13647-13652, 2002.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.