Anti-RPL13A Antibody Picoband® (monoclonal, 4C9)

60S ribosomal protein L13ais aproteinthat in humans is encoded by theRPL13Agene. It is mapped to 19q13.33. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L13P family of ribosomal proteins that is a component of the 60S subunit. The encoded protein also plays a role in the repression of inflammatory genes as a component of the IFN-gamma-activated inhibitor of translation (GAIT) complex. This gene is co-transcribed with the small nucleolar RNA genes U32, U33, U34, and U35, which are located in the second, fourth, fifth, and sixth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed throughout the genome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Mediator of RNA polymerase II transcription subunit 15; Activator-recruited cofactor 105 kDa component; ARC105; CTG repeat protein 7a; Mediator complex subunit 15; Positive cofactor 2 glutamine; Q-rich-associated protein; PC2 glutamine; Q-rich-associated protein; TPA-inducible gene 1 protein; TIG-1; Trinucleotide repeat-containing gene 7 protein; MED15; ARC105; CTG7A; PCQAP; TIG1; TNRC7

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway.

1. Wool IG, Chan YL, Glück A (1996) . Structure and evolution of mammalian ribosomal proteins. Biochem. Cell Biol. 73 (11–12) : 933–947. 2. Price SR, Nightingale MS, Bobak DA, et al. (1993) . Conservation of a 23-kDa human transplantation antigen in mammalian species. Genomics. 14 (4) : 959–964. 3. Nicoloso M, Qu LH, Michot B, Bachellerie JP (1996) . Intron-encoded, antisense small nucleolar RNAs: the characterization of nine novel species points to their role as guides for the 2'-O-ribose methylation of rRNAs. J. Mol. Biol. 260 (2) : 178–195.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.