Anti-Hsp90 alpha Antibody Picoband® (monoclonal, 6B5)

Heat shock protein HSP 90-alpha is a protein that in humans is encoded by the HSP90AA1 gene. The gene, HSP90AA1, encodes the human stress-inducible 90-kDa heat shock protein alpha (Hsp90A) . Complemented by the constitutively expressed paralog Hsp90B which shares over 85% amino acid sequence identity, Hsp90A expression is initiated when a cell experiences proteotoxic stress. Once expressed Hsp90A dimers operate as molecular chaperones that bind and fold other proteins into their functional 3-dimensional structures. This molecular chaperoning ability of Hsp90A is driven by a cycle of structural rearrangements fueled by ATP hydrolysis. Current research on Hsp90A focuses in its role as a drug target due to its interaction with a large number of tumor promoting proteins and its role in cellular stress adaptation.

Widely expressed at low level. Expressed in heart, brain, skeletal muscle, placenta, pancreas, spleen, prostate, mall intestine, colon, peripheral blood, leukocytes, bone marrow and fetal liver.Highly expressed in testes.

Probable Polycomb group (PcG) protein involved in transcriptional regulation mediated by ligand-bound nuclear hormone receptors, such as retinoic acid receptors (RARs) and peroxisome proliferator-activated receptor gamma (PPARG) .Acts as coactivator of RARA and RXRA through association with NCOA1.Acts as corepressor through recruitment of KDM1A and CBX5 to target genes in a cell-type specific manner; the function seems to involve differential recruitment of methylated histone H3 to respective promoters.Acts as corepressor for PPARG and suppresses its adipocyte differentiation-inducing activity (By similarity) .Non-catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1) .

1. Chen B, Piel WH, Gui L, Bruford E, Monteiro A (Dec 2005) . The HSP90 family of genes in the human genome: insights into their divergence and evolution. Genomics86 (6) : 627–37. 2. Hickey E, Brandon SE, Smale G, Lloyd D, Weber LA (Jun 1989) . Sequence and regulation of a gene encoding a human 89-kilodalton heat shock protein. Molecular and Cellular Biology 9 (6) : 2615–26.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.