Anti-SGT1/ECD Antibody Picoband®

Protein SGT1 is a protein that in humans is encoded by the ECD gene. Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes. Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins. The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex. May play a role in regulation of pre-mRNA splicing.

Disintegrin and metalloproteinase domain-containing protein 10; ADAM 10; CDw156; Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease; CD156c; ADAM10; KUZ; MADM

Apoptosis, Cancer, Cell Biology, Cell Death, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Receptors, Signal Transduction

Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (PubMed:20592283) . Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP) (PubMed:26686862, PubMed:11786905) . Contributes to the normal cleavage of the cellular prion protein (PubMed:11477090) . Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity (PubMed:12475894) . Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis (By similarity) . Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form (PubMed:17557115) . Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B (PubMed:19114711, PubMed:21288900) . May regulate the EFNA5-EPHA3 signaling (PubMed:16239146) .

1. Hartz, P. A. Personal Communication. Baltimore, Md. 7/9/2015. 2. Kim, J. H., Gurumurthy, C. B., Band, H., Band, V. Biochemical characterization of human Ecdysoneless reveals a role in transcriptional regulation. Biol. Chem. 391: 9-19, 2010. 3. Sato, T., Jigami, Y., Suzuki, T., Uemura, H. A human gene, hSGT1, can substitute for GCR2, which encodes a general regulatory factor of glycolytic gene expression in Saccharomyces cerevisiae. Molec. Gen. Genet. 260: 535-540, 1999.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.