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Anti-Astrin/Deepest/SPAG5 Antibody Picoband®

Boster Bio Anti-Astrin/Deepest/SPAG5 Antibody Picoband® catalog # A07062-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Sperm-associated antigen 5 is a protein that in humans is encoded by the SPAG5 gene. This gene encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functional and dynamic regulation of mitotic spindles.

Synonyms

Mimecan; Osteoglycin; Ogn; Og

Gene Name

SPAG5

Gene ID

10615

UniProt

Q96R06

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Astrin/Deepest/SPAG5 recombinant protein (Position: E738-S1193) .

Clonality

Polyclonal

Tissue Specificity

Expressed in kidney.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Cell Biology, Cell Cycle, Cell Differentiation, Cytoskeleton/ECM, Extracellular Matrix, Signal Transduction, Structures

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Induces bone formation in conjunction with TGF-beta-1 or TGF-beta-2.

References & Citations

1. Chang, M.-S., Huang, C.-J., Chen, M.-L., Chen, S.-T., Fan, C.-C., Chu, J.-M., Lin, W.-C., Yang, Y.-C. Cloning and characterization of hMAP126, a new member of mitotic spindle-associated proteins. Biochem. Biophys. Res. Commun. 287: 116-121, 2001. 2. Cheng, T.-S., Hsiao, Y.-L., Lin, C.-C., Yu, C.-T. R, Hsu, C.-M., Chang, M.-S., Lee, C.-I., Huang, C.-Y. F., Howng, S.-L., Hong, Y.-R. Glycogen synthase kinase 3-beta interacts with and phosphorylates the spindle-associated protein astrin. J. Biol. Chem. 283: 2454-2464, 2008. 3. Du, J., Jablonski, S., Yen, T. J., Hannon, G. J. Astrin regulates Aurora-A localization. Biochem. Biophys. Res. Commun. 370: 213-219, 2008.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

130 kDa, 150 kDa

Applications Notes

6

Gene Name Synonym

Osteoglycin

Subcellular Location

Secreted, extracellular space, extracellular matrix.

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

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