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Anti-Raptor/RPTOR Antibody Picoband®

Boster Bio Anti-Raptor/RPTOR Antibody Picoband® catalog # A01463-3. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Regulatory-associated protein of mTOR also known as raptor or KIAA1303 is an adapter protein that is encoded in humans by the RPTOR gene. This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms

Glutathione S-transferase A1; GST HA subunit 1; GST class-alpha member 1; GST-epsilon; GSTA1-1; GTH1; Glutathione S-transferase A1, N-terminally processed; GSTA1

Gene Name

RPTOR

Gene ID

57521

UniProt

Q8N122

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Raptor/RPTOR recombinant protein (Position: R301-R358) .

Clonality

Polyclonal

Tissue Specificity

Liver.

Applications

WB, ELISA

Field of Research

Epigenetics and Nuclear Signaling, Neurogenesis, Neurology Process, Neuroscience, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

References & Citations

1. Aylett, C. H. S., Sauer, E., Imseng, S., Boehringer, D., Hall, M. N., Ban, N., Maier, T. Architecture of human mTOR complex 1. Science 351: 48-52, 2016. 2. Capon, F., Helms, C., Veal, C. D., Tillman, D., Burden, A. D., Barker, J. N., Bowcock, A. M., Trembath, R. C. Genetic analysis of PSORS2 markers in a UK dataset supports the association between RAPTOR SNPs and familial psoriasis. (Letter) J. Med. Genet. 41: 459-460, 2004. 3. Hara, K., Maruki, Y., Long, X., Yoshino, K., Oshiro, N., Hidayat, S., Tokunaga, C., Avruch, J., Yonezawa, K. Raptor, a binding partner of target of rapamycin, mediates TOR action. Cell 110: 177-189, 2002.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

25722 MW

Observed Molecular Weight

150 kDa

Gene Name Synonym

Glutathione S-transferase alpha 1/2/3/4/5

Subcellular Location

Cytoplasm.

Protein Name

Glutathione S-transferase A1

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

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