Anti-PRDM14 Antibody Picoband® Fluoro550 Conjugated
Product Specifications
Background
This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer.
Synonyms
AF4/FMR2 family member 4; ALL1-fused gene from chromosome 5q31 protein; Protein AF-5q31; Major CDK9 elongation factor-associated protein; AFF4; AF5Q31, MCEF; HSPC092
Gene Name
PRDM14
Gene ID
63978
UniProt
Q9GZV8
Host
Rabbit
Reactivity
Human, Mouse, Rat
Cross Reactivity
No cross-reactivity with other proteins.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human PRDM14, which shares 55.2% amino acid (aa) sequence identity with mouse PRDM14.
Clonality
Polyclonal
Tissue Specificity
Ubiquitously expressed. Strongly expressed in heart, placenta, skeletal muscle, pancreas and to a lower extent in brain. .
Applications
Flow Cytometry
Field of Research
Epigenetics and Nuclear Signaling, Transcription, Cell Biology, Transcription Factors, Cell Cycle
Purification
Immunogen affinity purified.
Form
Liquid
Function
Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression. .
References & Citations
1. Chia, N.-Y., Chan, Y.-S., Feng, B., Lu, X., Orlov, Y. L., Moreau, D., Kumar, P., Yang, L., Jiang, J., Lau, M.-S., Huss, M., Soh, B.-S., Kraus, P., Li, P., Lufkin, T., Lim, B., Clarke, N. D., Bard, F., Ng, H.-H. A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. Nature 468: 316-320, 2010. 2. Jiang, G.-L., Huang, S. The yin-yang of PR-domain family genes in tumorigenesis. Histol. Histopath. 15: 109-117, 2000. 3. Nakaki, F., Hayashi, K., Ohta, H., Kurimoto, K., Yabuta, Y., Saitou, M. Induction of mouse germ-cell fate by transcription factors in vitro. Nature 501: 222-226, 2013.
Storage Conditions
At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Calculated Molecular Weight
127459 MW
Applications Notes
6
Gene Name Synonym
AF4/FMR2 family member 4
Subcellular Location
Nucleus . Associates to transcriptionally active chromatin but not at snRNA genes. .
Protein Name
AF4/FMR2 family member 4
Isotype
Rabbit IgG
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
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