Anti-Podoplanin/gp36/Pdpn Antibody Picoband®

Podoplanin (PDPN) is a protein that in humans is encoded by the PDPN gene. This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The International radiation Hybrid mapping consortium mapped the T1A gene to chromosome 1. It has got 162 amino acid protein which is a typical type I membrane glycoprotein, with an N-terminal signal sequence, a signal sequence cleavage site, a C-terminal hydrophobic stretch, and a short cytoplasmic tail. Northern blot analysis detected a 1.2-kb T1A2 transcript only in lung. It has been described as a differentiation antigen and influenza-virus receptor, and a marker of lung injury.

Podoplanin; Aggrus; Glycoprotein 36; Gp36; PA2.26 antigen; T1-alpha; T1A; 29kDa cytosolic podoplanin intracellular domain; PICD; PDPN; GP36; PSEC0003, PSEC0025

Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas.

Cancer, Co-Activators/Co-Repressors, Energy Metabolism, Energy Transfer Pathways, Epigenetics and Nuclear Signaling, Metabolic Signaling Pathways, Metabolism, Neurology Process, Neuroscience, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling, Nuclear Signaling Pathways, Pathways and Processes, Signal Transduction, Signaling Pathway

Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation (PubMed:14522983, PubMed:15231832, PubMed:17616532, PubMed:18215137, PubMed:17222411) . Interaction with CD9, on the contrary, attenuates platelet aggregation induced by PDPN (PubMed:18541721) . Through MSN or EZR interaction promotes epithelial-mesenchymal transition (EMT) leading to ERZ phosphorylation and triggering RHOA activation leading to cell migration increase and invasiveness (PubMed:17046996, PubMed:21376833) . Interaction with CD44 promotes ional cell migration in epithelial and tumor cells (PubMed:20962267) . In lymph nodes (LNs), controls fibroblastic reticular cells (FRCs) adhesion to the extracellular matrix (ECM) and contraction of the actomyosin by maintaining ERM proteins (EZR; MSN and RDX) and MYL9 activation through association with unknown transmembrane proteins. Engagement of CLEC1B by PDPN promotes FRCs relaxation by blocking lateral membrane interactions leading to reduction of ERM proteins (EZR; MSN and RDX) and MYL9 activation (By similarity) . Through binding with LGALS8 may participate to connection of the lymphatic endothelium to the surrounding extracellular matrix (PubMed:19268462) . In keratinocytes, induces changes in cell morphology showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion (PubMed:15515019) . Controls invadopodia stability and maturation leading to efficient degradation of the extracellular matrix (ECM) in tumor cells through modulation of RHOC activity in order to activate ROCK1/ROCK2 and LIMK1/LIMK2 and inactivation of CFL1 (PubMed:25486435) . Required for normal lung cell proliferation and alveolus formation at birth (By similarity) . Does not function as a water channel or as a regulator of aquaporin-type water channels (PubMed:9651190) . Does not have any effect on folic acid or amino acid transport (By similarity) .

1. Zimmer G, Oeffner F, Von Messling V, Tschernig T, Groness HJ, Klenk HD, Herrler G (Sep 1999) . Cloning and characterization of gp36, a human mucin-type glycoprotein preferentially expressed in vascular endothelium. Biochem J 341 (Pt 2) : 277–84. PMC 1220357. PMID 10393083. 2. Ma T, Yang B, Matthay MA, Verkman AS (Jul 1998) . Evidence against a role of mouse, rat, and two cloned human t1alpha isoforms as a water channel or a regulator of aquaporin-type water channels. Am J Respir Cell Mol Biol 19 (1) : 143–9. PMID 9651190. 3. Entrez Gene: PDPN podoplanin.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.