Anti-RHOF Antibody Picoband® Fluoro647 Conjugated

Rho, the Ras-related small GTPase, is responsible for the regulation of actin-based cytoskeletal structures including stress fibers, focal adhesions and the contractile ring apparatus. Rho proteins function as molecular switches that are able to turn cytokinesis on and off. Although little is known about signaling downstream of Rho, a host of putative Rho effector proteins have been described. Rho F (ras homolog gene family, member F), also known as RIF or ARHF, is a 211 amino acid membrane protein that localizes to the cytoplasmic side of the plasma membrane. Belonging to the small GTPase superfamily and the Rho family, Rho F functions cooperatively with Cdc42 and Rac to generate additional cytoskeletal structures, such as increasing variation of actin-based morphology. Rho F exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 12q24.31.

1. Ellis, S., Mellor, H. The novel Rho-family GTPase Rif regulates coordinated actin-based membrane rearrangements. Curr. Biol. 10: 1387-1390, 2000. 2. Fan, L., Pellegrin, S., Scott, A., Mellor, H. The small GTPase Rif is an alternative trigger for the formation of actin stress fibers in epithelial cells. J. Cell Sci. 123: 1247-1252, 2010. 3. Fan, L., Yan, H., Pellegrin, S., Morigen, Mellor, H. The Rif GTPase regulates cytoskeletal signaling from plexinA4 to promote neurite retraction. Neurosci. Lett. 590: 178-183, 2015.