Anti-Transketolase/TKT Picoband® Antibody (monoclonal, 2I3)

Transketolase is a thiamine-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway. The pentose phosphate pathway, which is active in most tissues, provides sugar phosphates for intermediary biosynthesis, especially nucleotide metabolism, and generates the biosynthetic reducing power for the cell in the form of NADPH. Transketolase is ly involved in the branch of the pathway that channels excess sugar phosphates to glycolysis, enabling the production of NADPH to be maintained under different metabolic conditions. NADPH is critical for maintaining cerebral glutathione, and thus it is likely that transketolase plays an important role in brain metabolism.

ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.

1. Abedinia, M., Layfield, R., Jones, S. M., Nixon, P. F., Mattick, J. S. Nucleotide and predicted amino acid sequence of a cDNA clone encoding part of human transketolase. Biochem. Biophys. Res. Commun. 183: 1159-1166, 1992. 2. Boyle, L., Wamelink, M. M. C., Salomons, G. S., Roos, B., Pop, A., Dauber, A., Hwa, V., Andrew, M., Douglas, J., Feingold, M., Kramer, N., Saitta, S., Retterer, K., Cho, M. T., Begtrup, A., Monaghan, K. G., Wynn, J., Chung, W. K. Mutations in TKT are the cause of a syndrome, including short stature, developmental delay, and congenital heart defects. Am. J. Hum. Genet. 98: 1235-1242, 2016. 3. Coy, J. F., Dubel, S., Kioschis, P., Thomas, K., Micklem, G., Delius, H., Poustka, A. Molecular cloning of tissue-specific transcripts of a transketolase-related gene: implications for the evolution of new vertebrate genes. Genomics 32: 309-316, 1996.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.