Pyruvate Fluorometric Assay Kit
Product Specifications
Type
Fluorescence method
Field of Research
Glycolysis and Lipid Metabolism; Tricarboxylic Acid (TCA) Cycle
Assay Principle
Pyruvate is an important component of the organism. It mainly participates in the metabolism of sugar and fat, and is also one of the intermediate products of carbohydrate metabolism. The detection principle of this kit is that pyruvate undergoes a series of reactions under the action of enzyme reagents, and the resulting substance can react with the color developer. The content of pyruvate in the sample is calculated by measuring the fluorescence value of excitation wavelength 535 nm and emission wavelength 587 nm.
Assay Performance Time
40 min
Sample Type
Serum; Plasma; Urine; Animal tissue; Cell
Detection Range
1.00-100 μmol/L
Sensitivity
1.00 μmol/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Inter CV
2.0-4.0%
Intra CV
1.0-2.0%
Detection Instrument
Fluorescence microplate reader
Available Sizes
Documents
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