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Anti-Grainyhead-like protein 1/GRHL1 Antibody Picoband®

Boster Bio Anti-Grainyhead-like protein 1/GRHL1 Antibody Picoband® catalog # A07368-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

This gene encodes a member of the grainyhead family of transcription factors. The encoded protein can exist as a homodimer or can form heterodimers with sister-of-mammalian grainyhead or brother-of-mammalian grainyhead. This protein functions as a transcription factor during development.

Synonyms

Troponin I, fast skeletal muscle; Troponin I, fast-twitch isoform; TNNI2

Gene Name

GRHL1

Gene ID

29841

UniProt

Q9NZI5

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Grainyhead-like protein 1/GRHL1 recombinant protein (Position: M1-I618) .

Clonality

Polyclonal

Tissue Specificity

Highly expressed in brain and weakly in heart, small intestine and uterus. Isoform 1A is mostly expressed in granular cell and molecular layer. Isoform 1B is mostly expressed in Purkinje cells. Isoform 1E is predominantly expressed in peripheral tissues as kidney, lung, trachea, colon, small intestine, stomach, bone marrow, thymus and mammary gland. .

Applications

ELISA, Flow Cytometry, IF, ICC, WB

Field of Research

Interleukins

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

References & Citations

1. Huang, N., Miller, W. L. Cloning of factors related to HIV-inducible LBP proteins that regulate steroidogenic factor-1-independent human placental transcription of the cholesterol side-chain cleavage enzyme, P450scc. J. Biol. Chem. 275: 2852-2858, 2000. 2. Jacobs, J., Atkins, M., Davie, K., Imrichova, H., Romanelli, L., Christiaens, V., Hulselmans, G., Potier, D., Wouters, J., Taskiran, I. I., Paciello, G., Gonzalez-Blas, C. B., Koldere, D., Aibar, S., Halder, G., Aerts, S. The transcription factor grainy head primes epithelial enhancers for spatiotemporal activation by displacing nucleosomes. Nature Genet. 50: 1011-1020, 2018.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

108320 MW

Observed Molecular Weight

70 kDa

Applications Notes

6

Gene Name Synonym

Troponin I2, fast skeletal type

Subcellular Location

Troponin complex. Cytosol. Nucleus.

Protein Name

Gamma-aminobutyric acid type B receptor subunit 1

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.

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