Anti-N WASP/WASL Antibody Picoband®

Neural Wiskott-Aldrich syndrome protein is a protein that in humans is encoded by the WASL gene. This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension.

Cell Type Marker, Developmental Families, Domain Families, Epigenetics and Nuclear Signaling, Neurogenesis, Neurology Process, Neuroscience, Transcription

Multifunctional transcription factor with different regions mediating its different effects. Acts by binding (via its C-terminal domain) to sequences related to the consensus octamer motif 5'-ATGCAAAT-3' in the regulatory regions of its target genes. Regulates the expression of specific genes involved in differentiation and survival within a subset of neuronal lineages. It has been shown that activation of some of these genes requires its N-terminal domain, maybe through a neuronal-specific cofactor. Ativates BCL2 expression and protects neuronal cells from apoptosis (via the N-terminal domain) . Induces neuronal process outgrowth and the coordinate expression of genes encoding synaptic proteins. Exerts its major developmental effects in somatosensory neurons and in brainstem nuclei involved in motor control. Stimulates the binding affinity of the nuclear estrogene receptor ESR1 to DNA estrogen response element (ERE), and hence modulates ESR1-induced transcriptional activity. May positively regulate POU4F2 and POU4F3. Regulates dorsal root ganglion sensory neuron specification and axonal projection into the spinal cord. Plays a role in TNFSF11-mediated terminal osteoclast differentiation. Negatively regulates its own expression interacting ly with a highly conserved autoregulatory domain surrounding the transcription initiation site.

1. Cotta-de-Almeida, V., Westerberg, L., Maillard, M. H., Onaldi, D., Wachtel, H., Meelu, P., Chung, U., Xavier, R., Alt, F. W., Snapper, S. B. Wiskott-Aldrich syndrome protein (WASP) and N-WASP are critical for T cell development. Proc. Nat. Acad. Sci. 104: 15424-15429, 2007. 2. Fukuoka, M., Miki, H., Takenawa, T. Identification of N-WASP homologs in human and rat brain. Gene 196: 43-48, 1997. 3. Guenin-Mace, L., Veyron-Churlet, R., Thoulouze, M.-I., Romet-Lemonne, G., Hong, H., Leadlay, P. F., Danckaert, A., Ruf, M.-T., Mostowy, S., Zurzolo, C., Bousso, P., Chretien, F., Carlier, M.-F., Demangel, C. Mycolactone activation of Wiskott-Aldrich syndrome proteins underpins Buruli ulcer formation. J. Clin. Invest. 123: 1501-1512, 2013.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.