Anti-Gsta3 Antibody Picoband® Fluoro647 Conjugated

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class genes that are located in a cluster mapped to chromosome 6. Genes of the alpha class are highly related and encode enzymes with glutathione peroxidase activity. However, during evolution, this alpha class gene diverged accumulating mutations in the active site that resulted in differences in substrate specificity and catalytic activity. The enzyme encoded by this gene catalyzes the double bond isomerization of precursors for progesterone and testosterone during the biosynthesis of steroid hormones. An additional transcript variant has been identified, but its full length sequence has not been determined.

A synthetic peptide corresponding to a sequence at the N-terminus of mouse Gsta3, which shares 57.9% and 89.5% amino acid (aa) sequence identity with human and rat Gsta3, respectively.

Expressed in the brain, thymus, and tonsils. Expressed in samples from patients with chronic myeloid leukemia (CML) and in 58% of acute myeloid leukemia (AML) cell lines.

1. Board, P. G. Identification of cDNAs encoding two human alpha class glutathione transferases (GSTA3 and GSTA4) and the heterologous expression of GSTA4-4. Biochem. J. 330: 827-831, 1998. 2. Morel, F., Rauch, C., Coles, B., Le Ferrec, E., Guillouzo, A. The human glutathione transferase alpha locus: genomic organization of the gene cluster and functional characterization of the genetic polymorphism in the hGSTA1 promoter. Pharmacogenetics 12: 277-286, 2002. 3. Suzuki, T., Johnston, P. N., Board, P. G. Structure and organization of the human alpha class glutathione S-transferase genes and related pseudogenes. Genomics 18: 680-686, 1993.