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Anti-Cyclophilin E/PPIE Antibody Picoband®

Selected Variant

A08021-1

Size: 100 µg/Vial

Boster Bio Anti-Cyclophilin E/PPIE Antibody Picoband® catalog # A08021-1. Tested in ELISA, IP, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Peptidylprolyl isomerase E (cyclophilin E), also known as PPIE, is an enzyme which in humans is encoded by the PPIE gene on chromosome 1. The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 1, has been identified.

Synonyms

BMP and activin membrane-bound inhibitor homolog; Non-metastatic gene A protein; Putative transmembrane protein; NMA; BAMBI; NMA

Gene Name

PPIE

Gene ID

10450

UniProt

Q9UNP9

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Cyclophilin E/PPIE recombinant protein (Position: M1-V301) .

Clonality

Polyclonal

Tissue Specificity

Expressed in adult liver.

Applications

WB, ICC, IF, IP, Flow Cytometry, ELISA

Field of Research

Atherosclerosis, Cardiovascular, Chaperones, Heat Shock Proteins, Protein Trafficking, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Negatively regulates TGF-beta signaling.

References & Citations

1. Kim, J.-O., Nau, M. M., Allikian, K. A., Makela, T. P., Alitalo, K., Johnson, B. E., Kelley, M. J. Co-amplification of a novel cyclophilin-like gene (PPIE) with L-myc in small cell lung cancer cell lines. Oncogene 17: 1019-1026, 1998. 2. Mi, H., Kops, O., Zimmermann, E., Jaschke, A., Tropschug, M. A nuclear RNA-binding cyclophilin in human T cells. FEBS Lett. 398: 201-205, 1996.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

17136 MW

Observed Molecular Weight

35 kDa

Applications Notes

6

Gene Name Synonym

BMP and activin membrane bound inhibitor

Subcellular Location

Cytoplasm . Nucleus . Translocates to nuclear foci during heat shock.

Protein Name

Chromaffin granule amine transporter

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

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