Anti-EIF2AK4 Antibody Picoband® Fluoro647 Conjugated
Product Specifications
Background
EIF2AK4 (Eukaryotic Translation Initiation Factor 2-Alpha Kinase 4), also called GCN2, is an enzyme that in humans is encoded by the EIF2AK4 gene. EIF2AK4 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 to downregulate protein synthesis in response to varied cellular stresses. Hartz (2005) mapped the EIF2AK4 gene to chromosome 15q15.1 based on an alignment of the EIF2AK4 sequence with the genomic sequence. Berlanga et al. (1999) demonstrated that Gcn2 immunopurified from mouse liver extracts could phosphorylate rabbit Eif2 in vitro. Serum starvation increased the level of phosphorylated EIF2-alpha more than 2-fold in human embryonic kidney cells transfected with mouse Eif2ak4. Costa-Mattioli et al. (2005) reported a unique feature of hippocampal slices from Gcn2-null mice: in CA1, a single 100-Hz train induced a strong and sustained long-term potentiation (late LTP or L-LTP), which was dependent on transcription and translation.
Synonyms
Placenta growth factor; PlGF; PGFL; PLGF; PGF
Gene Name
EIF2AK4
Gene ID
440275
UniProt
Q9P2K8
Host
Rabbit
Reactivity
Human, Monkey, Mouse
Cross Reactivity
No cross-reactivity with other proteins.
Immunogen
E.coli-derived human EIF2AK4 recombinant protein (Position: Q23-K440) .
Clonality
Polyclonal
Tissue Specificity
While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.
Applications
Flow Cytometry
Field of Research
Growth Factors/Hormones, Metabolism, Metabolism Processes, Pathways and Processes, Response To Hypoxia, Signal Transduction
Purification
Immunogen affinity purified.
Form
Liquid
Function
Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth.
References & Citations
1. Berlanga, J. J., Santoyo, J., de Haro, C. Characterization of a mammalian homolog of the GCN2 eukaryotic initiation factor 2-alpha kinase. Europ. J. Biochem. 265: 754-762, 1999. 2. Costa-Mattioli, M., Gobert, D., Harding, H., Herdy, B., Azzi, M., Bruno, M., Bidinosti, M., Ben Mamou, C., Marcinkiewicz, E., Yoshida, M., Imataka, H., Cuello, A. C., Seidah, N., Sossin, W., Lacaille, J.-C., Ron, D., Nader, K., Sonenberg, N. Translational control of hippocampal synaptic plasticity and memory by the elF2-alpha kinase GCN2. Nature 436: 1166-1170, 2005. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 3/28/2005.
Storage Conditions
At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Applications Notes
6
Gene Name Synonym
Placental growth factor
Subcellular Location
Secreted.
Isotype
Rabbit IgG
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
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