Anti-CYP11B1/C11B2/CYP11B2 Antibody Picoband® Fluoro647 Conjugated

CYP11B1 shows close homology to the CYP11B2 gene, which encodes aldosterone synthase and is normally expressed only in the zona glomerulosa. Both genes map to chromosome 8q21. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane.

NAD-dependent protein lipoamidase sirtuin-4, mitochondrial; NAD-dependent ADP-ribosyltransferase sirtuin-4; NAD-dependent protein deacetylase sirtuin-4; Regulatory protein SIR2 homolog 4; SIR2-like protein 4; SIRT4; SIR2L4

Detected in vascular smooth muscle and striated muscle. Detected in insulin-producing beta-cells in pancreas islets of Langerhans (at protein level) . Widely expressed. Weakly expressed in leukocytes and fetal thymus.

Acetylation, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Chromatin Modifying Enzymes, Diabetes, Diabetes-associated, Energy Metabolism, Energy Transfer Pathways, Epigenetics and Nuclear Signaling, Heart Disease, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications. Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest. In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor. Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis. Does not seem to deacetylate PC. Controls fatty acid oxidation by inhibiting PPARA transcriptional activation. Impairs SIRT1:PPARA interaction probably through the regulation of NAD+ levels. Down-regulates insulin secretion.

1. Curnow, K. M., Slutsker, L., Vitek, J., Cole, T., Speiser, P. W., New, M. I., White, P. C., Pascoe, L. Mutations in the CYP11B1 gene causing congenital adrenal hyperplasia and hypertension cluster in exons 6, 7, and 8. Proc. Nat. Acad. Sci. 90: 4552-4556, 1993. 2. Clyne, C. D., Zhang, Y., Slutsker, L., Mathis, J. M., White, P. C., Rainey, W. E. Angiotensin II and potassium regulate human CYP11B2 transcription through common cis-elements. Molec. Endocr. 11: 638-649, 1997. 3. Ganapathipillai, S., Laval, G., Hoffman, I. S., Castejon, A. M., Nicod, J., Dick, B., Frey, F. J., Frey, B. M., Cubeddu, L. X., Ferrari, P. CYP11B2-CYP11B1 haplotypes associated with decreased 11-beta-hydroxylase activity. J. Clin. Endocr. Metab. 90: 1220-1225, 2005.