Anti-REC8 Antibody Picoband®

Meiotic recombination protein REC8 homolog is a protein that in humans is encoded by the REC8 gene. It is mapped to 14q12. This gene encodes a member of the kleisin family of SMC (structural maintenance of chromosome) protein partners. The protein localizes to the axial elements of chromosomes during meiosis in both oocytes and spermatocytes. In the mouse, the homologous protein is a key component of the meiotic cohesion complex, which regulates sister chromatid cohesion and recombination between homologous chromosomes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.

Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.

1. Brar, G. A., Kiburz, B. M., Zhang, Y., Kim, J.-E., White, F., Amon, A. Rec8 phosphorylation and recombination promote the step-wise loss of cohesins in meiosis. Nature 441: 532-536, 2006. 2. Parisi, S., McKay, M. J., Molnar, M., Thompson, M. A., van der Spek, P. J., van Drunen-Schoenmaker, E., Kanaar, R., Lehmann, E., Hoeijmakers, J. H. J., Kohli, J. Rec8p, a meiotic recombination and sister chromatid cohesion phosphoprotein of the Rad21p family conserved from fission yeast to humans. Molec. Cell. Biol. 19: 3515-3528, 1999. 3. Xu, H., Beasley, M. D., Warren, W. D., van der Horst, G. T. J., McKay, M. J. Absence of mouse REC8 cohesin promotes synapsis of sister chromatids in meiosis. Dev. Cell 8: 949-961, 2005.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.