Anti-CD31/PECAM1 Antibody Picoband® (monoclonal, 2D4)

CD31 also known as Platelet endothelial cell adhesion molecule (PECAM-1), is a protein that in human is encoded by the PECAM1 gene. Encoded protein is a member of the immunoglobulin superfamily, CD31 is mapped to 17q23.3. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is demonstrated that CD31 expression on human PBSCs may positively affect both neutrophil and platelet engraftment. Meanwhile, CD31 is involved in leukocyte migration and angiogenesis, which are key components of venous thrombus resolution.

Adhesion molecule antibody; CD31 antibody; CD31 antigen antibody; CD31 EndoCAM antibody; Endocam antibody; FLJ34100 antibody; FLJ58394 antibody; GPIIA antibody; GPIIA' antibody; PECA1 antibody; PECA1_HUMAN antibody; Pecam 1 antibody; PECAM 1 CD31 EndoCAM antibody; PECAM antibody; PECAM-1 antibody; Pecam1 antibody; Platelet endothelial cell adhesion molecule antibody; Platelet/endothelial cell adhesion molecule 1 antibody; Platelet/endothelial cell adhesion molecule antibody

E.coli-derived human CD31 recombinant protein (Position: Q28-G382) . Human CD31 shares 65% and 68% amino acid (aa) sequences identity with mouse and rat CD31, respectively.

Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Expressed in human umbilical vein endothelial cells (HUVECs) (at protein level) . Expressed on neutrophils (at protein level) . Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level) .

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis. Does not protect against apoptosis.

1. Newman PJ, Berndt MC, Gorski J, White GC, Lyman S, Paddock C, Muller WA (March 1990) . PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily. Science 247 (4947) : 1219–22. 2. Gumina RJ, Kirschbaum NE, Rao PN, vanTuinen P, Newman PJ (June 1996) . The human PECAM1 gene maps to 17q23. Genomics 34 (2) : 229–32. 3. Xie Y, Muller WA (October 1996) . Fluorescence in situ hybridization mapping of the mouse platelet endothelial cell adhesion molecule-1 (PECAM1) to mouse chromosome 6, region F3-G1. Genomics 37 (2) : 226–8. 4. Kellermair J, Redwan B, Alias S, Jabkowski J, Panzenboeck A, Kellermair L, Winter MP, Weltermann A, Lang IM.Platelet endothelial cell adhesion molecule 1 deficiency misguides venous thrombus resolution.2013 Nov 7; 122 (19) :3376-84. 5. Donmez A, Tombuloglu M, Gulbahar O, Arik B, Cagirgan S, Vural F, Gokmen N.CD31 expression on peripheral blood stem cells predicts both early neutrophil and platelet engraftments.2013 Oct;49 (2) :307-12.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.