Anti-XLF/NHEJ1 Picoband® Antibody Fluoro594 Conjugated

Non-homologous end-joining factor 1 (NHEJ1), also known as Cernunnos or XRCC4-like factor (XLF), is a protein that in humans is encoded by the NHEJ1 gene. It is mapped to 2q35. Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.

DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V (D) J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary. Binds DNA in a length-dependent manner.

1. Callebaut, I., Malivert, L., Fischer, A., Mornon, J.-P., Revy, P., de Villartay, J.-P. Cernunnos interacts with the XRCC4-DNA-ligase IV complex and is homologous to the yeast nonhomologous end-joining factor Nej1. J. Biol. Chem. 281: 13857-13860, 2006. 2. Cantagrel, V., Lossi, A.-M., Lisgo, S., Missirian, C., Borges, A., Philip, N., Fernandez, C., Cardoso, C., Figarella-Branger, D., Moncla, A., Lindsay, S., Dobyns, W. B., Villard, L. Truncation of NHEJ1 in a patient with polymicrogyria. Hum. Mutat. 28: 356-364, 2007. 3. Dai, Y., Kysela, B., Hanakahi, L. A., Manolis, K., Riballo, E., Stumm, M., Harville, T. O., West, S. C., Oettinger, M. A., Jeggo, P. A. Nonhomologous end joining and V (D) J recombination require an additional factor. Proc. Nat. Acad. Sci. 100: 2462-2467, 2003.